Progress was measured and fingerprints of chlorogenic acid in honeysuckle extract
Conceptual: The substance of chlorogenic corrosive in the honeysuckle bloom lately was explored and the fingerprints of herbs were evaluated, which gave the reference to future research.
Honeysuckle for honeysuckle plants, honeysuckle gonad honeysuckle, or hamster honeysuckle dry buds or with early blooms. The medication is normally utilized as a part of customary Chinese prescription, chlorogenic corrosive is the fundamental practical segments of honeysuckle, its adequacy relies on upon the level of chlorogenic corrosive. Chlorogenic corrosive is caffeoylquinic corrosive subordinates, dissolvable in ethanol, CH3)2CO, somewhat solvent in ethyl acetic acid derivation, is a critical physiological dynamic substance, it has hostile to bacterial, against infection, antipyretic, hoisted white platelets, Liver rummaging, resistant control, hostile to tumor, bringing down circulatory strain, bringing down blood fat, searching free radicals et cetera. In this paper, the creator outlines the exploration advance of fingerprints of honeysuckle and its primary dynamic fixing chlorogenic corrosive substance assurance strategy, which can give a reference to future research.
1.Determination of chlorogenic corrosive substance
Notwithstanding the Chinese Pharmacopeia test techniques, has been accounted for investigation strategies are bright spectrophotometry, subsidiary spectroscopy, thin layer chromatography, hindrance of a synergist blurring spectrophotometry, fine zone electrophoresis, elite fluid chromatography (HPLC) and so forth.
1.1 Ultraviolet spectrophotometry
Li Na et al.  set up a fast assurance technique for chlorogenic corrosive substance in honeysuckle remove. The strategy was exact, quick and advantageous. The ideal wavelength of the chlorogenic corrosive was 294 (R = 0.995 2, n = 1), which can be utilized for the on-line quality control of honeysuckle concentrate in the creation of Qingkailing infusion. The outcomes demonstrate that there is a decent connection between’s the deliberate esteem and the anticipated estimation of chlorogenic corrosive ; Due to the chlorogenic corrosive atoms containing phenolic hydroxyl, Zhang Qiufang 1 According to phenolic mixes phenolic hydroxyl and ferric chloride shading response, found that chlorogenic corrosive and FEC 1, the shading response result of the optical thickness and green The substance of chlorogenic corrosive was higher than that of HPLC, however the distinction was <1O%, which was most likely because of the distinction of chlorogenic corrosive in honeysuckle There will be a couple of different substances will be with the shading reagent happened in the shading response, proposing that in functional applications, ought to be founded on various zones of honeysuckle fixings and the level of the quick assurance of the consequences of such an important remedy.
1.2 Differential Derivative Spectroscopy
Lin Liming I31 found that sodium tungstate as the explanatory reagent can rapidly respond with the aggregate chlorogenic corrosive to create edifices. As per the trademark changes of the range, the differential subsidiary spectrophotometry can be utilized to decide the honeysuckle and its planning the aggregate substance of chlorogenic corrosive in detoxification sheet. The normal recuperation rate was 100.4% and the coefficient of variety was 1.82%. The strategy is straightforward, quick and precise.
1.3 subsidiary polarography
LI Bai-lin Studies on the electrochemical conduct and chlorogenic corrosive substance of chlorogenic corrosive in citrus extract were contemplated by straight checking second-arrange subsidiary polarography. It was found that in NH-NHCl medium, chlorogenic corrosive was temperamental, Resulting in a bigger examination mistake. In the sodium citrate medium, the honeysuckle filtering arrangement and silver yellow infusion of chlorogenic corrosive substance assurance, to acquire agreeable outcomes.
1.4 Thin layer chromatography
Xu Qiang et al. Assurance of chlorogenic corrosive in honeysuckle in the pharyngeal pocket by thin layer checking technique. Subsequent to examining with a thin layer scanner, it was resolved that trichloromethane-ethyl acetic acid derivation monocarboxylic corrosive (2: 2: 1 The technique was basic and exact. The strategy was additionally used to decide the chlorogenic corrosive in honeysuckle by utilizing the thin-layer filtering technique. The examples were portrayed by X-beam diffraction (XRD) and examining electron microscopy (SEM) The extraction conditions demonstrated that methanol, 70% ethanol could make the spots simple to diffuse, and the ethanol extraction impact was better. Contrasted and the thin layer conditions, the chloroform-ethyl acetic acid derivation formic corrosive (4: 4: 2) Good division impact, sawtooth reflection technique double wavelength examining, 325nm, R4OOnm, checking speed 20ram/rain, opening 1.25ram × 1.25ram, recorder affectability × 2, straight parameters × 3; The outcomes demonstrated that the substance of chlorogenic corrosive was controlled by thin layer filtering strategy, which gave another strategy to the quality control of honeysuckle and its readiness. The chlorogenic corrosive was isolated and measured by polyamide film, and the chromatographic pinnacle Shape well, wipe out Tailing chlorogenic corrosive on the silica gel thin layer.
1.5 Inhibitory a reactant blurring spectrophotometry
In the hydrochloric corrosive medium, press (ⅲ) can catalyze the response of hydrogen peroxide to reestablish brilliant green SF, and chlorogenic corrosive has a touchy severe impact on the synergist response. Geng Yuzhen et al. (1) set up a strategy for the assurance of follow chlorogenic corrosive Inhibitory a synergist active spectrophotometry. The straight scope of chlorogenic corrosive was 0-0.12mg/L, and as far as possible was 4.2 × 10g/L. The relative standard deviation (RSD) of the standard arrangement of 0.08 mg/L chlorogenic corrosive was 3.8% and the recuperation was 93.4% ~ 105%. The ideal conditions and energy of the response were additionally researched. The technique was connected to the assurance of chlorogenic corrosive in honeysuckle draining arrangement, and palatable outcomes were acquired.
1.6 Capillary Zone Electrophoresis
Hairlike ice examination has the upsides of high determination, quick investigation speed, and condition kind disposition. As of late, hairlike electrolysis has turned into an expansive and powerful investigative system in the exploration of conventional Chinese prescription quality. (64.5cm × 50m, powerful length 56cm) was utilized as the division channel, 30mmol • L borax (pH = 9.3) was utilized as the running cradle answer for the treatment of honeysuckle by microwave-helped extraction. The division temperature of 20RV; hairlike section temperature 25 ℃; recognition wavelength 340 nm (chlorogenic corrosive) and 270nm (rutin, quercetin) in the meantime partition and assurance of honeysuckle rutin, chlorogenic corrosive and quercetin substance, comprehensive and successful control of honeysuckle restorative quality to give a straightforward, quick technique; Long Hong I1. etc, a narrow zone electrophoretic investigation strategy for the assurance of chlorogenic corrosive markers in eight exclusive Chinese prescriptions of honeysuckle and honeysuckle was proposed. The foundation electrolyte utilized was a support arrangement of pH 7.00 containing lOmmol/L phosphate and 20retool/L borate and contained 5% ethanol to build the solvency of the example. In the direct scope of 27.765 ~ 665ug/mL, the relationship coefficient r 0.9996. Assurance of honeysuckle and honeysuckle 8 sorts of exclusive Chinese pharmaceutical in the chlorogenic corrosive can be finished inside 15rain. This investigation strategy is straightforward, quick, extensive variety of uses.
1.7Superior Liquid Chromatography
The fundamental strategy for the assurance of chlorogenic corrosive in honeysuckle is the superior fluid chromatography. (20: 8O, v/v); stream rate: 0.8 ml/min, recognition wavelength: 327nm, infusion volume: 1Ol; segment temperature: room temperature, The substance of chlorogenic corrosive in honeysuckle were thought about on the ODS section. The outcomes demonstrated that the substance of chlorogenic corrosive in honeysuckle was higher than that in Huixian County, (15: 85: 0.2) as the portable stage, and the stream rate was 0%. The substance of chlorophyllic corrosive was lower than that of the control amass (P <0.05), and the substance of chlorophyllic corrosive was lower in Linyi territory, Linyi range, The outcomes demonstrated that when the grouping of cold acidic corrosive was higher than that of icy acidic corrosive, the outcomes demonstrated that when the centralization of icy acidic corrosive was higher than that of chilly acidic corrosive in the compelling region of honeysuckle, 0.2%, we can show signs of improvement pinnacle shape; Wang Tianzhi and other I1 1 set up by turn around stage elite fluid chromatography inner standard slope elution, and assurance of honeysuckle chlorogenic corrosive, caffeic corrosive, 3,5 twelve caffeoylquinic corrosive in the strategy, and three sorts of starting point 4 Yinhua the over three mixes were given half investigation. Techniques: The angle elution was done in various extents of Shimadzu CLC-ODS section (6.0ram × 150ram), methanol-10% disodium hydrogen phosphate and acidic corrosive cushion versatile stage. The inside standard was salicylic corrosive and the identification wavelength was 304nm The outcomes demonstrated that the substance of chlorogenic corrosive, caffeic corrosive, and 3,5-caffeic corrosive was higher than that of Shandong and Henan honeysuckle. The outcomes demonstrated that the substance of chlorogenic corrosive, caffeic corrosive, and 3,5-caffeic corrosive was higher than that of Shandong and Henan honeysuckle. This strategy is touchy quick, exact and solid.
The relative maintenance time and a relative pinnacle territory of every segment in the therapeutic materials under the given chromatographic conditions are exceptional to every segment, for example, the human unique finger impression, utilizing the relative maintenance time and the relative pinnacle zone to set up the relative maintenance of the specimen Value of the unique mark. A progression of parameters, including n solid pinnacle, trademark crest gathering, and trademark unique mark top recognition rate, can be delivered by the information of the chromatogram of the specimen, which can give a target premise to the ID and separation of the nature of Chinese home grown drug. At present, the fingerprints of honeysuckle are examined by GC, HPLC, HPCE et cetera.
2.1 GC strategy
(15m × 0.22mm); section temperature 90 ~ C starting temperature, to 4 ℃/min to 200 ~ ~ C, 40 ~ C/min rose to 240 ~ C to keep up; Carrier gas: high virtue nitrogen 1.2ml/min; make up gas: nitrogen 40ml/min, split proportion 1:10; hydrogen 40 ml/min; air 500 ml/Min; indicator FID; identification chamber 245 ℃; gasification chamber 235 ℃, the chromatographic unique mark of honeysuckle herbs was established.The technique depended on linalool as the reference material, and 11 clusters of honeysuckle were dissected and found that 24 tops The outcomes demonstrated that the chromatographic pinnacles of honeysuckle were great, and the chromatographic pinnacles of honeysuckle were great. The pinnacle territory is 1, ascertain the relative maintenance time and pinnacle region proportion of the other regular unique mark crests, and compute the pinnacle range proportion distinction of the pinnacle region of over 10% of the aggregate pinnacle zone, and acquire the specialized parameters of honeysuckle gas stage fingerprints. The outcomes demonstrate that the honeysuckle gas stage Fingerprint dependability, great reproducibility, solid attributes, The range proportion and its distinction, non-basic pinnacle region are in accordance with the arrangements of the consequences of steady and dependable, can be utilized as one of the ID criteria for infusion of honeysuckle herbs.Wang Bin, “the utilization of GC chromatography to set up a unique mark of conventional Chinese prescription honeysuckle, and comparable Some issues in the use of programming are examined. Strategy: SGE 30QC3 quartz slim section (30m × 0.32ram × 0.5 “1TI); segment temperature: 100 ~ C at typical temperature, rose to 1 50 ℃ at 2 ℃/min, 5 ℃/1TIin rose to 1 70 Nitrogen, 40 ml/min, split proportion 1:10; hydrogen, 40 ml/min; air, 400 ml/min; Detector: FID; Detector temperature: 200 ~ C; Gasification chamber temperature: 200 ~ C. From this technique, the GC model of herbs was set up, and the similitude of various clusters was thought about. Great detachment can be utilized as the unique mark of the herbs.
2.2 HPCE technique
Rongong et al. Utilized the technique for high-effectiveness hairlike electrophoresis to build up HPCE fingerprints of honeysuckle from various zones with 40 mm L/L borax arrangement as the cushion. Quartz hairlike: 57 cm x 75 m (viable length 50 cm), working voltage 17 kV, temperature 25 ° C, weight infusion time 6 S, identification wavelength: 214 nm. Through the grouping examination of the trademark information of fingerprints, the restorative materials of various territories were partitioned into three classes, and the discriminant capacity was built up for a wide range of tests. Utilizing the unique finger impression method, the recognizable proof and quality control of the honeysuckle restorative materials in various regions can be made by bunch examination or the foundation of discriminant capacity. Sun Guoxiang et al., 11 7I, utilizing fine precipitation technique with 50 mmol/L borax (containing 20 mmol/L (PH 8.5) was utilized as the foundation electrolyte, working voltage 12 kV, UV discovery wavelength 254 nm, gravity infusion l5 s (stature 8.5 cm), the foundation of the honeysuckle therapeutic water separate slim electrophoresis Fingerprints. The pinnacle fingerprints of honeysuckle were dictated by the rates of the electrophoretic pinnacle of 100%, and the technique had great exactness and reproducibility. Sex, detachment effectiveness and ease.
2.3 HPLC technique
Bai Xuemei et al. I 1 utilized HPL C to set up the unique finger impression of honeysuckle. The chromatographic conditions were as per the following: segment YGW-C18 (2 50mm x 4.6mm, 10 m), identification wavelength 238nm, section temperature 30 ℃, versatile stage: A fluid 0.4% phosphoric corrosive watery arrangement, The measure of the specimens was 20 l, and the elution was done. The parts contained in the four honeysuckles were assessed and the chlorogenic corrosive substance was resolved. The outcomes demonstrated that there were no huge contrasts in the substance of chlorogenic corrosive between the fundamental segments of honeysuckle and the control herbs (p> 0.05). The chlorogenic corrosive was the reference material, The foundation of HPLC fingerprinting strategy for methanol concentrate of 10 groups of honeysuckle delivered in Shandong from 2003 to 2004 was gathered. The chromatographic section was ODS, (4.6mm × 250mm, 5 1TI), versatile stage: acetonitrile: 0.4% phosphoric corrosive (15:85), segment temperature: 3 0 ℃, stream rate: 1 1TIl/1TIi n, identification wavelength : 307nm. The outcomes for the herbs have 15 basic pinnacles, the parameters of “Chinese medication infusion fingerprints specialized necessities” of the applicable arrangements, can be utilized as honeysuckle quality assessment and species Kam cut one of the vital premise; Wang Qian [l] angle elution strategy, The honeysuckle restorative materials were controlled by HPLC. The portable stage comprised of 1% acidic corrosive watery arrangement and B stage was eluted with methanol. The stream rate was 1.0 mL/ml. The identification was completed with the accompanying equation: Z0RBAX SB-C18 (150mm × 4.6mm, 5m) Wavelength: 254rim; Column temperature: 30 ° C. The HPLC fingerprints have great reproducibility, and 1 trademark fingerprints of l0 clumps of tests have specificity. The honeysuckle therapeutic technique set up in this review has the attributes of HPLC, Fingerprint examination can be utilized to assess the nature of honeysuckle