Five common methods for the detection of plant extracts
There are five common methods for the detection of plant extracts:
- High-Performance Liquid Chromatography (HPLC)
- Ultraviolet spectrophotometer (UV)
- Thin layer chromatography (TLC)
- Gas Chromatography (GC)
- Atomic Absorption Spectrometry (AAS)
HPLC and UV are commonly used as standard plant extracts. TLC is used for the detection of proportional extracts. GC is used to detect volatile liquids or oils. AAS is used for the detection of heavy metals in extracts.
1.High-performance liquid chromatography (HPLC)
High-performance liquid chromatography (HPLC) is a mobile phase with liquid as a mobile phase. A high-pressure infusion system is used to pump a mobile solution with a single solvent or different proportions of mixed solvents, buffers and the like into a column with a stationary phase. After the components are separated, they enter the detector for detection, so as to achieve the analysis of the sample. The method has become an important separation and analysis technology in the fields of chemistry, medicine, industry, agriculture, commodity inspection and forensic examination.
Our company’s products are basically high-performance liquid chromatography.
High-performance liquid chromatography content calculation:
Internal standard method: a more accurate quantitative method in chromatographic analysis, especially in the absence of a standard control, this method is more superior to its superiority. The internal standard method is to add a certain weight of pure substance as an internal standard (see the internal standard material) to a certain amount of the sample mixture to be analyzed, and then the sample containing the internal standard for chromatographic analysis of the internal standard and The peak area (or peak height) of the component to be measured and the relative correction factor, according to the formula and method can be obtained in the sample composition of the percentage of the sample
Area normalization method
Assume that all the components in the sample flow out and the detector generates a signal for them to calculate the impurity peak area and the sum.
2.Ultraviolet absorption spectroscopy (UV)
UV detection method uses some substances to absorb the radiation of 10 ~ 800nm spectral region of the method of analysis and determination, widely used in organic and inorganic substances qualitative and quantitative determination. The method has the characteristics of high sensitivity, good accuracy, good selectivity, simple analysis and good speed of analysis.
The UV absorption spectrum of the substance is essentially a characteristic of the chromophore and chromophore in its molecule, not the whole molecule.
3.Thin Layer Chromatography (TLC)
A suitable stationary phase is applied to a glass plate, plastic or aluminum substrate to form a uniform layer. (Rf) for the identification, impurity inspection or content determination of the drug, according to the ratio (Rf) of the appropriate control to the chromatogram obtained by the same method The Thin layer chromatography is a very important experimental technique for rapid separation and qualitative analysis of a small amount of material
4.Gas Chromatography (GC) Gas as a mobile phase chromatography.
5.Atomic Absorption Spectrometry (AAS)
It is an analytical method based on the absorption intensity of the corresponding atomic resonance radiation of ultraviolet and visible light in the outer layer of the ground state atoms of the gaseous state. The method is mainly applicable Analysis of Trace and Trace Components in Samples.