Parkinson disease (PD) is a common middle-aged nervous system disease, the etiology is not clear. And honokiol (HL) is a bioactive substance-containing phenolic hydroxyl group extracted from Magnolia officinalis, which has the effects of scavenging free radicals and protecting nerve cells. The author used the neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MP1rP) to make the model of Parkinson’s disease in order to elucidate the protective effect of honokiol on neurons and possible The mechanism of action.
1 Materials and methods
1.1 Experimental material C57BL / 6 male mice 60, weight 20 ~ 25g, by the Academy of Military Medical Sciences Animal Experimental Center. And Magnolol (Plantnat Natural Ingredients Inc. www.plantnat.com)
1.2.1 Animal model establishment and grouping The mice were randomly divided into 5 groups (n = 12), control group, MP, RP model group, amantadine group (positive control, 40mg / kg) And honokiol A (10 mg / kg), and magnolol B (30 mg / kg). Amantadine and honokiol are preventive administration, continuous gavage 14d. In the other four groups, the other four groups were given MPTP 30mg / kg intraperitoneally 1 hour before the administration of the administration, and the behavioral indexes were given for 1 day after the last administration. The rats were sacrificed at 4 ℃ and stored at -80 ℃ for the determination of dopamine (DA) in the tissues. Determine the success index of modeling: mice appear temporary torso tremor, vertical hair, tail overstretched, action reduction and another involuntary movement.
1.2.2 General behavior observed in mice observed in the abdominal cavity after the general behavior of MPTP modeling, with or without abnormal response, compare the differences between the groups.
1.2.3 Independent activity experiments The spontaneous activity of mice was measured and counted using the XZ24 mouse autonomous instrument. Mice were placed in a self-active box (13 cm high, 25 cm in diameter, 4 mice at a time, 1 in each activity box). The mice were recorded automatically from the recorder. A number of activities .
1.2.4 DA content determination of high-performance liquid-electrochemical method (HPLC_EC) determination of striatum DA content.
1.3 Statistical analysis of measurement data as well as ± s, by SPSS 13.0 statistical software processing. There was a significant difference between the two groups using single factor analysis of variance (P <0.05).
2.1 general behavior observed intraperitoneal administration of MPTP (30mg / kg) 5min, the mice appear temporary torso tremor, vertical hair, tail extension, action reduction and another involuntary movement, proved Parkinson model established successfully. Compared with the control group, the general behavior of the mice in the MPTP model group was abnormal. Most of the following changes were observed: tailing, vertical hair, increased saliva secretion, increased breathing, decreased muscle tension and tooth tremor. The duration was generally 3- 4h. And the honokiol A group, B group and amantadine group, the symptoms were mild, shorter duration.
(P <0.01). Compared with MPTP model group, the number of autonomous activities in the group of Magnolol A and B was significantly increased by JJI1 (P <0.01), and the number of autonomic activities in the MPTP model group was significantly lower than that in the control group (P <0.05). There was no significant difference in the number of autonomous activities between amantadine group and honokiol A and B groups (P> 0.05, Table 1).
2.3 DA content MPTP model group compared with the control group, DA content was significantly reduced (P <0.01). (P <0.05). The DA content of honokiol A, B group, and amantadine group was significantly higher than that of model group (PO.05), indicating that pretreatment with honokiol could increase the content of DA in striatum (Table 1)
MPTP modeling is currently the most commonly used method of preparing Parkinson’s disease animal model. Monoamine oxidase B catalyzes MPTP into MPP (+), which inhibits mitochondrial oxidative respiratory chain, produces reactive oxygen and nitric oxide, leading to dopaminergic neuronal necrosis and apoptosis, resulting in animals producing symptoms similar to Parkinson’s disease And pathological changes. The results of this study show that MPTP can induce mice in the behavioral aspects of muscle tremors, random exercise increased, decreased the number of independent activities similar to the characteristics of Parkinson’s disease.
Magnolol (magnolo1), and honokiol is the traditional active ingredient of traditional Chinese medicine Magnolia officinalis, the clinical application of anti-anxiety, antidepressant, anti-morphine withdrawal reaction, inhibition of catecholamine secretion and so on. Studies have shown that 0.2mg / kg or greater doses of honokiol are not only anxiolizable, but also non-stable side effects . And honokiol by antagonizing Na and Ca. Plasma intracellular flow, thereby inhibiting the acetylcholine-induced adrenal pheochromocytoma catecholamine secretion.
This study found that honokiol can improve the behavioral symptoms of Parkinson’s disease in rats with varying degrees of improvement in DA levels in the striatum and improve autonomy. Suggesting that the mechanism may be related to the role of honokiol and helminthic neurons, promoting DA neuron survival, differentiation, and growth, and thus part of the recovery of DA synthesis and metabolism.
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