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Analysis of Extraction Methods of Sapindus Active Ingredients (Saponins)

Analysis of Extraction Methods of Sapindus Active Ingredients (Saponins)

OBJECTIVE: To establish the best method for the extraction of sapindo saponin, which can be put into industrial production. It requires simple extraction method, low cost and high yield.

At present, there are 6 methods of extracting the active ingredient:

1.Ultrafiltration

2.enzymatic

3.Microwave extraction

4.Ultrasonic method

5.bubble separation method

6.Methanol, anhydrous ethanol, water extraction

 

Extraction method analysis and comparison:

  1. Ultrafiltration

The saphenous fruit peel washed, dried, crushed, extracted with water three times, combined with water extract, adding chitosan flocculant impurity, the flocculation of water extract after ultrafiltration separation, permeate at 60 ℃ Off-white powder is the product.

  1. Enzyme

Wash the aseptic peel, dry, crush. Accurately weighed a certain amount of Sapindus powder, with distilled water as a solvent to 1: 5 solid-liquid ratio placed in a three-necked flask. Plus enzyme after constant temperature reflux extraction for a certain time. The extract was subjected to high temperature sterilization and then flocculated. The saponin content in the extract were determined by UV – Vis spectrophotometry.

 

  1. Microwave extraction

Pre-treatment → water pretreatment → ethanol extraction → ethanol removal → acid hydrolysis → neutralization → water removal → extraction agent extraction → extraction agent removal → drying → products.

  1. Ultrasonic method

Take 15 grams of water into the conical flask, add water 250ml, using ultrasonic extraction method for 50min, ultrasonic power of 100W, the temperature is room temperature (in the extraction process, the water temperature rise 14 ℃ or so, the temperature of the ultrasonic extraction of organic matter results do not affect); extraction is completed, then add the same amount of Sapindus, the ultrasonic extractor in the warm water into cold water, ultrasonic extraction 50min; Finally, the filtrate was removed by suction filtration to obtain a concentrated extract of sapindus.

  1. Foam separation method

Foam Separator Figure 1 is a schematic diagram of an experimental device for batch foam separation. The foam separation column has an inner diameter of 47 mm and a height of 700 mm. After the air is compressed by the compressor, the flow rate is controlled by the rotor flowmeter, and the gas is distributed through the gas distributor, and the crude extract of sapindus saponins is introduced from the bottom of the column. The foam rises along the column and reaches the top of the column through the foam spill into the foam collection device, which continues until the resulting foam can not overflow from the unit. The volume of the collected liquid was measured and the saponin content was measured by spectrophotometry after drying.

  1. Methanol, anhydrous ethanol, water extraction method

Take 100.0g of pediatric peel, add 500 ml jar, soak with industrial methanol (methanol over peels), soak for 4d. Methanol and Sapindus poured into the tissue crusher mashed, cotton gauze filter, and hand squeezed out of the mashed material in the remaining methanol, the residue and soaked in fresh methanol for 2 d. Cotton gauze filter, hand squeeze out the mashed material in the remaining methanol, methanol solution merger. Plus 100g non-sashimi peel, repeat the above steps, the merger of methanol solution. The batch components were concentrated at 45 ° C under reduced pressure to no methanol and the concentrate was combined. Anhydrous ethanol and water extraction methods and procedures and methanol extraction methods and steps the same.

Comparison of Extraction Methods of Six Kinds of Sapindus Active Ingredients

 

Suggested method: enzymatic

The optimum conditions were as follows: the amount of cellulase was 0.1% of the mass of the sapenite powder, the time of the enzyme was 25h, the temperature of the enzyme was 50 ℃ and the pH was 4.7. At this time, the extraction rate of sapindus saponins reached 86.59%, 19.63% higher than that without enzyme treatment. Reagents and instruments Reagents: hydrochloric acid, sodium hydroxide, vanillin, glacial acetic acid, perchloric acid and so on. ; Cellulase (enzyme activity> 15000u / g), amylase (enzyme activity> 10000u / g), pectinase (enzyme activity> 50u / g)

 

To know more about saponin tech data, benefits, function and application. Where to buy saponin?

Megan Sum2017-08-15T09:51:34+00:00

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