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  • rosemary extract extract

3 ways of Extraction Technology of Rosmarinic Acid

 

3 ways of Extraction Technology of Rosmarinic Acid

Roseminic acid (C18H16O8), chemical name [R (E)] α – [[3- (3,4-dihydroxyphenyl) -l-oxo-2-propenyl] oxy] (3-oxo-2E-pro-penyl] oxy] -3,4-dihydroxy-benzenepropanoicacid), (3,4-dihydroxy-benzenepropanoicacid) Chemical structure shown in Figure 1. Rosmarinic acid is phenolic compounds, light yellow powder, a melting point of 202 ~ 205 ° C, soluble in water, ethanol, methanol, insoluble in chloroform, ether, and other organic solvents.

Rosmarinic acid was isolated from rosemary by Ellis in 1958 and distributed extensively in plants. It mainly exists in the family of Labiatae, Lithospermate, Cucurbitaceae, Tiliaceae, Umbelliferae Among a variety of plants. Rosmarinic acid is a natural water-soluble antioxidant, with a variety of biological activity, widely used in food, medicine, and cosmetics. In this paper, the extraction and separation methods of rosmarinic acid and quantitative analysis methods are reviewed to provide a valuable reference for the production and application of rosmarinic acid.

  1. Solvent extraction method

solvent extraction method is the medicine in the appropriate solvent at room temperature or soaking conditions for a certain period of time, leaching of active ingredients of a method. This method is easy to operate, easy. The extraction temperature was 65 ℃, the extraction time was 3h, the ratio of material to liquid was 1:50 (w). The extraction temperature was 65 ℃, the extraction time was 3h, the ratio of material to liquid was 1:50 (w) / V) and rosmarinic acid yield of 23.63 mg / g. (50% ethanol) as extractant, the ratio of material to liquid was 1:50 (w / v), the extraction temperature was 65 ℃, the extraction time was 35min, the content of rosmarinic acid The extraction rate was 1.061%, which was 30.18% higher than that of hot water extraction.

rosemary-planting-base

  1. Ultrasonic extraction method

Rosematic acid extraction is the most commonly used method for ultrasonic extraction. Ultrasonic extraction method using ultrasonic extraction of active ingredients. The principle of the use of ultrasonic cavitation, destruction of medicinal cells, so that the solvent easily penetrate into the cell, while the strong vibration of ultrasound can transfer huge energy to the extraction of herbs and solvents to make high-speed movement to strengthen the release of intracellular substances, Diffusion, and dissolution, to accelerate the leaching of active ingredients.

The extraction process of rosmarinic acid in Salvia miltiorrhiza Bunge was studied by ultrasonic extraction. The effects of four factors on the ratio of material to a liquid, the concentration of methanol, the extraction temperature and the extraction time were orthogonal design under single factor and single level, And the content of rosmarinic acid was extracted from Salvia miltiorrhiza as the validation index of Salvia miltiorrhiza. The results showed that the extraction efficiency was the best when the feed/liquid ratio was 1: 25, 50% methanol was extracted at 75 ℃ for 30 min. CHEN Ling-ling, ZHU De-Quan et al.

Extraction of rosmarinic acid from Radix Astragali Sinensis by ultrasonic extraction method. The optimum extraction conditions were as follows: 20 times 30% ethanol ultrasonic extraction was carried out for 40 minutes. Extraction of rosmarinic acid from Prunella vulgaris by ultrasonic extraction. Extraction technology was ultrasonic time 1h, extraction solvent 70% ethanol, ultrasonic power 400W, a ratio of solid to liquid 1:30. Zou Shengqin, Sun Xiaoqing The extraction of rosmarinic acid from rosemary was carried out by ultrasonic extraction. (40% ethanol) as extraction agent, the ratio of material to liquid was 1:60 (m / V), the ultrasonic output power was 300W, the extraction time was 15min, and the mixture was extracted from the basil. The yield of the acid was 1.146%. The results showed that the ultrasonic pretreatment time was 10 min, and 15% ethanol was the best method for the extraction of rosmarinic acid from rosemary by ultrasonic assisted heat recovery method. The results showed that the ultrasonic pretreatment time was 10 min, Solvent, extraction time is 1h, under which the extraction rate of rosmarinic acid is 94.94%.

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  1. Supercritical fluid extraction

Supercritical fluid extraction is a new technology that utilizes the fluid formed by a substance in the supercritical region and extracts the active ingredient in the plant. Supercritical fluid means that when a substance is at its critical temperature and above the critical pressure, the formation of a non-liquid and non-gas in a special state, in this state, the fluid both gas-liquid two-phase characteristics, both with the gas Similar concentrations, but also close to the density of liquid, for many substances have a strong ability to dissolve, can be used as extraction solvent. The extraction conditions were extraction pressure 40MPa, extraction temperature 65 ℃, extraction time 1h, entrainer (ethanol) dosage 0.60ml (rhodium) • G-1.

Plantnat Natural Ingredients Inc. has made rosemary extract for more than 12 years , if you need any rosemary acid , please try to contact with us by : sales@plantnat.com

rosemary acid

rosemary acid

rosemary acid

Megan Sum2017-05-26T02:18:26+00:00

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